Continuous Culture of the Postimplantation Rat Conceptus 1
نویسندگان
چکیده
منابع مشابه
Chloroquine embryotoxicity in the postimplantation rat conceptus in vitro.
The embryotoxicity of the antimalarial drug chloroquine (CQ) was evaluated in vitro using the rat whole embryo culture system. CQ was found to be embryotoxic and dysmorphogenic when added directly to the culture media containing gestational day (GD) 10 rat conceptuses. Twenty-six-hr exposure to CQ elicited dose-related decreases in embryonic crown-rump length, protein and DNA contents and incre...
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Rat postimplantation whole-embryo culture (WEC) is a promising alternative test for the assessment of developmental toxicity. Toxicogenomic-based approaches may improve the predictive ability of the WEC model by providing a means to identify compound-specific mechanistic responses associated with embryotoxicity in vivo. Furthermore, alterations in gene expression may serve as a sensitive, objec...
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Rat conceptuses of pregnancy day 11 (embryonic age approximately 10-5 days) with embryos within the yolk and amniotic sacs and polar allantoic placental tissues were cultured by the 'New system' utilizing homologous serum in 60 ml roller bottles with 20% O2, 5% CO2 and 75% N2 gas environment (five conceptuses/10 ml serum). Embryonic growth and development was assessed by external morphology and...
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Luteotrophic activity of the young conceptus was investigated on the basis of the maintenance ofpregnancy produced artificially by means of egg transfer to the uteri of adult `cycling' rats treated with progesterone for 5 to 11 days. Pregnancy rate varied according to the duration ofprogesterone treatment; the longer the treatment, the higher the rate of success. Luteotrophic activity of the co...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1984
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod31.2.415